| CTRP9

C1q and Tumor Necrosis Factor Related Protein 9 (CTRP9) is an adiponectin paralog that has been identified as possessing significant biological activities. CTRP9, also known as C1QTNF9, C1QTNF9A, AQL1, or Complement C1q and tumor necrosis factor-related protein 9A, is a member of the C1q/tumor necrosis factor (TNF)-related protein family. It is an adipose-derived cytokine that shares similar metabolic regulatory functions with adiponectin.CTRP9 exhibits a range of biological properties, including anti-inflammatory, antioxidant, vasodilatory, and atheroprotective effects. It has been shown to inhibit apoptosis of endothelial cells, decrease blood glucose levels, and increase insulin sensitivity.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.28	1.22	0.74	0.52	0.31	0.24	0.19	0.08
Corrected OD	2.2	1.14	0.66	0.44	0.23	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human CTRP9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human CTRP9 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108